Request QuoteCatalog Number: xP742039PIJSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP742039PIJYeast1mgQuote
EP742039PIJE. coli1mgQuote
BP742039PIJBaculovirus200ugQuote
MP742039PIJMammalian Cell200ugQuote

Protein Information

SpeciesPhotorhabdus luminescens subsp. laumondii (strain TT01)
UniProt IDQ7MB63
Gene NamemiaB; Locus:plu1312
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-476
Expression Tag6xHis
Purity>90%
AA SequenceMSTIKKLYIKTWGCQMNEYDSSKMADLLESTHGYQLTDVAEEADILLLNTCSIREKAQEK VFHQLGRWKNLKDTNPDLIIGVGGCVASQEGDFIRQRAQCVDIIFGPQTLHRLPEMINQV KGTRSPVVDISFPEIEKFDRLPEPRAEGPSAFVSIMEGCNKYCTFCVVPYTRGEEVSRPC DDVLFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELIRLVAAIDGIDRIRFTT SHPIEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTLMKRAHTALEYKSIIRKLRKARP DILISSDFIIGFPGETQDDFEKTMKLIADVNFDMSYSFIYSARPGTPAADLPDDVSEEEK KQRLYLLQQRINQQAMSYSRAMLGSVQRILVEGTSRKNVMELSGRTENNRVVNFEGQPDM IGKFVDVEIVDVYANSLRGKVIRTEDQMDLRIHESPESVIARTRKEDEIGVGTYQP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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