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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP762733WBQYeast1mgQuote
EP762733WBQE. coli1mgQuote
BP762733WBQBaculovirus200ugQuote
MP762733WBQMammalian Cell200ugQuote

Protein Information

SpeciesWolinella succinogenes
UniProt IDQ7MR25
Gene NamemiaB; Locus:WS1776
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-433
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLFIQTLGCAMNVRDSEHMIAELEAKEGYTLTDDPKEADLILINTCSVREKPERKLF SEIGQFSKEKKEEAKIGVCGCTASHLGSEILKKAPSVSFVLGARNVSKISRVIHQEKAVE VATDYDDSSYVFATGSRNDYKAMVNISIGCDKKCAYCIVPHTRGQEISVPSDLILGEARR LASAGVKEILLLGQNVNHYGRRFSSAHPKISFTELLRELSQVEGIERLRFTSPHPLHMDD EFLEEFASNPKICKSIHMPLQSGSTRILSLMRRGYSQEWFINRVERLKALAPETTIGTDI IVGFPGESEEDFLGTMEVLERVRFETLYSFVYSPRPHTEAASWENLVDEEVASERLHRLQ ARHKEILEELNQKEVGAIHSVLWEHQRRDEGWCEGRTDTGKMVRMKGGEEFLGRITPVRI KEAYRAFLIGEPL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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