Request QuoteCatalog Number: xP745630GCISize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP745630GCIYeast1mgQuote
EP745630GCIE. coli1mgQuote
BP745630GCIBaculovirus200ugQuote
MP745630GCIMammalian Cell200ugQuote

Protein Information

SpeciesGloeobacter violaceus (strain PCC 7421)
UniProt IDQ7NE65
Gene NamemiaB; Locus:glr4015
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-441
Expression Tag6xHis
Purity>90%
AA SequenceMESTTNPQGKTACLVTFGCQMNKADSERMAGALTHLGYRIVDESDTADLVLFNTCTIRDN AEQKVYSYLGQQARRKQRDPHITLVLAGCVAQQEGEKLLRRVPELDLVMGPQHVNRLSDL LERVAEGEQVVATEPIEILEDITKPRRDSAVTAWANIIYGCNEGCTYCIVPSVRGREQSR TPEAIKAEICELGASGYKEVTLLGQNIDAYGRDIGTNLASLLRFIHNAPGIERLRFATSH PRYFSDELIATCAELPKVCEHFHIPFQSGDNEVLRRMARGYTHEKYRAIIEKIRAILPDA AISADLIVGFPGETEAQFENSLRLVDELEFDALNTAAYSPRPGTPAARWPGQLDDEVKQD RLQRTNRLVAQKAFERSQRYLGRTEQVLVEETNPRDRSQVVGRTRTNRLVFFAGELAKLR GQLVDVQITAARAFSLTGTAS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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