Request QuoteCatalog Number: xP744629TNSSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP744629TNSYeast1mgQuote
EP744629TNSE. coli1mgQuote
BP744629TNSBaculovirus200ugQuote
MP744629TNSMammalian Cell200ugQuote

Protein Information

SpeciesThermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
UniProt IDQ72J39
Gene NamemiaB; Locus:TT_C0942
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-465
Expression Tag6xHis
Purity>90%
AA SequenceMPPCYPFLLSWEGWARDPPGRPGGAMRAHIITYGCQMNEYDSHLVASELVSLGWELVDSV EEADFVLVNTCAVRGKPVEKVRSLLGQLRKEKERRGLLIGMMGCLAQLDEGQQMAKKFGV DVLLGPGALTSLPEALKANERFFDLTFREDVLDYIPPPPKGALSAHVTIIRGCNHHCTYC IVPTTRGPEVSRHPDLILKEIELLKQAGVVEVTLLGQNVNSYGKDQPGFPSFAELLRMVG GMGIPRVRFLTSHPVNFTDDIIEAIAETPAICRYIHLPVQSGSDRVLRRMAREYRRAHYL ERIRKIREALPDAVLSTDIIVGFPGETEEDFQETLSLYDEVGYDQAYMFIYSPRPGTPAY KHFQDLPREVKVERLMRLIEKQKEWSYRRNLEWVGKTVEVLVRGEAKEEGFVQGHDRGNH PVLVPASQAPVPGLYQVEIKQATPHLLFGEVVGAEAPAPIPLPVA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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