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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP748157WAQYeast1mgQuote
EP748157WAQE. coli1mgQuote
BP748157WAQBaculovirus200ugQuote
MP748157WAQMammalian Cell200ugQuote

Protein Information

SpeciesWolbachia pipientis wMel
UniProt IDQ73HW8
Gene NamemiaB; Locus:WD_0421
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-439
Expression Tag6xHis
Purity>90%
AA SequenceMKGLYIKTYGCQMNVYDSVLMENVIKPLGFNVVSDAGKADLVILNTCHIREKAAEKLYSE LGKIHSLRKEMTIVVAGCVAQAEGEEVFRRAPFVDIVVGPQSIATLPELIVKASRSKGHV INTDFPEVAKFDKLPDECYGNSQGSSAFLAIQEGCDKFCTFCVVPYTRGAEYSRPVNEIF REALKLVANGANEINLLGQNVNAYHGECEGEVWDLGKLISHIAKIEKLERIRYTTSHPRD MHESLYLAHAEEPKLMPFVHLPVQSGSNKILHAMNRKHTAEEYLEIIDRFRKLKPEIEFS SDFIVGFPGETEKDFEETMKLVEKVRYAQAYSFKYSPRPGTPGAERKDQVPEEVKTERLL RLQKLISKQQLEFNQSMVGKTIPVLFSDKKGKHQNQIIGKSPYMQSVCIDDSEDKYRDKI VNVKVLEARQSSLLGCAFH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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