Request QuoteCatalog Number: xP672871DZRSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP672871DZRYeast1mgQuote
EP672871DZRE. coli1mgQuote
BP672871DZRBaculovirus200ugQuote
MP672871DZRMammalian Cell200ugQuote

Protein Information

SpeciesCupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha)
UniProt IDQ475N7
Gene NamemiaB; Locus:Reut_A0514
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-450
Expression Tag6xHis
Purity>90%
AA SequenceMKKVFVKTYGCQMNEYDSDKMVDVLNASQGLEPTDNVEDADVILFNTCSVREKAQEKVFS ELGRMKALKAVKPDLVIGVGGCVASQEGASIVSRAPYVDVVFGPQTLHRLPDLIARRQRT GQSQVDISFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDV LAEVAGLAEQGVREVTLLGQNVNAYRGAMGGTSEIADFALLIEYVAEIPGIERIRYTTSH PKEFTSRLVELYGRCDKLVNHLHLPVQHASDRVLMAMKRGYSVLEYKSIIRRLRMLRPDM SMSSDFIVGFPGETDADFDKLMALIEEVGYDTSFSFIFSPRPGTPAANLHDDTPHEVKLR RLQHLQATIEENVQRISRNMVGSVQRILVEGPSRKDPTELHGRTENNRVVNFALPGVPQA QRDRMIGQMVDVSITQAFPHSLRGEIVVRQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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