Request QuoteCatalog Number: xP690272PWESize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB)

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB (miaB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP690272PWEYeast1mgQuote
EP690272PWEE. coli1mgQuote
BP690272PWEBaculovirus200ugQuote
MP690272PWEMammalian Cell200ugQuote

Protein Information

SpeciesPseudomonas syringae pv. syringae (strain B728a)
UniProt IDQ4ZN97
Gene NamemiaB; Locus:Psyr_4345
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-442
Expression Tag6xHis
Purity>90%
AA SequenceMAKKLYIETHGCQMNEYDSSRMVDLLGEHQALEVTARAEDADVILLNTCSIRERAQDRVY SQLGRWRELKLANPEMVIAVGGCVASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDAARV TRLPQVDVSFPEIEKFDHLPEPRVDGPSAYVSVMEGCSKYCTFCVVPYTRGEEVSRPFDD VLSEVIHLAENGVREVTLLGQNVNGYRGTTHDGRVADLADLIRVVAAVDGIDRIRYTTSH PLEFSDSLIQAHAEVPELVKHLHLPVQSGSDRILAAMKRNHTTLEYKSRLRKLRAAVPGI SISSDFIVGFPGETEKDFDNTMKLIEDVGFDFSFSFVYSPRPGTPAADLKDDTPEALKKE RLAALQHRLNQQGFEISRQMVGSIQRILVTDYSKKDPGELQGRTENNRIVNFRCDNPKLI GQFADVHIDDAQPHSLRGSLLQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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