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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP455293BYIYeast1mgQuote
EP455293BYIE. coli1mgQuote
BP455293BYIBaculovirus200ugQuote
MP455293BYIMammalian Cell200ugQuote

Protein Information

SpeciesAmoebophilus asiaticus (strain 5a2)
UniProt IDB3ES11
Gene NamemiaB; Locus:Aasi_0614
Protein Name
Region Expressed1-486
Expression Tag6xHis
Purity>90%
AA SequenceMQDKVLTDLIKTEKPEQIEDFLPNEEDITSPTITRNLYVESYGCQMNIADSEVVVSILRP HGFEITDTYEKADVIFINTCAIRDKAEQTVRKRLSQFNQLKRRNPDLVIGVLGCMAERLK QTLLEEEKLVDLVAGPDAYRDLPRLLATVDSGHKAINTFLSREETYADISPIRLNSNGVS AFISIMRGCDNMCSFCVVPFTRGRERSRDPYSIVKEATELFEQGYREVTLLGQNVDSYKW SPNTDKKEQPTTPQEGVVNFAQLLAMVAQIHPDLRIRFSTSHPKDITDQVLYTIKAYDNI CKYIHLPVQSGSSRVLKLMNRTYDRAWYLQKIEDIRRIVGEDCGISSDMIAGFCTETEED HQDTLSLMEHIKYEFSYMFYYSERPGTLAARKYADDVPLAVKKRRLQEIIDKQRQHSFEK NLKDIGKVYQVLVEGPSKKSDIEWQGRNSANKVVVFPNVDCKKGTYVDVLITNCTTGTLL GEICTR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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