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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP425451TKEYeast1mgQuote
EP425451TKEE. coli1mgQuote
BP425451TKEBaculovirus200ugQuote
MP425451TKEMammalian Cell200ugQuote

Protein Information

SpeciesThermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / TMO)
UniProt IDA8F716
Gene NamemiaB; Locus:Tlet_1393
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-436
Expression Tag6xHis
Purity>90%
AA SequenceMRVFFKTYGCQMNLNDTETMAGILSQHGYEVVNLPEEADIVILNTCVVRQKSQEKYHSAL GQFVKLKKSGKIKLIGIAGCGSNLEGEELIKSGADFVIGSRSIGKIAEVLQKAARGEKIV YLEDDICTVDSKTPRMRFSKHHAWITIIHGCNRFCTYCIVPYTRGREKSRPLPDVLLEVE KLAKNGVKEITFLGQNVDAYGKDLKDGTNLASLIEQAGKFEQIKRIWFLTSYPTDITDKL IETVAEDPKAAKSFHIPVQSGSNRILRLMNRRYDRDQFLQLVEKIRSKIPHASISSDIIV GFPTETEYDYMQTMDLVRKARFERLNLAVYSPRQGTVASKYFKDDVPREEKVQRLNKLLE LQKQINRELNMQYLGKVVEIIVEGKTKEGLYYGRDIRNKVIIFSSQEVSEGENVLLKIDK ITAGPLYGKLQKKCGL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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