Request QuoteCatalog Number: xP539501AXHSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP539501AXHYeast1mgQuote
EP539501AXHE. coli1mgQuote
BP539501AXHBaculovirus200ugQuote
MP539501AXHMammalian Cell200ugQuote

Protein Information

SpeciesActinobacillus pleuropneumoniae serotype 3 (strain JL03)
UniProt IDB0BQR0
Gene NamemiaB; Locus:APJL_1339
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-475
Expression Tag6xHis
Purity>90%
AA SequenceMAKLHITTWGCQMNEYDSSKMADLLNSTHGLELTDKPEEADVLLLNTCSIREKAQEKVFS QLGRWKNWKKDKPDLIIGVGGCVASQEGEHIRDRAPFVDIVFGPQTLHRLPEMINKIRGG DRAIVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCSFCVVPYTRGEEVSRPVDDV LFEIAQLAEQGVREVNLLGQNVNAYRGETFDGGICTFAELLRLVAAIDGIDRVRYTTSHP IEFTDDIIEVYRDTPELVSFLHLPIQSGADRVLTMMKRNHTALEYKAIIRKLREVRPNIQ ISSDFIVGFPGETAEDFEQTMKVIEQVNFDMSFSFIYSARPGTPAADLPDDISEEEKKAR LARLQQRINHQAMQFSRAMLGTEQRVLVEGPSKKDIMELTGRTENNRIVNFQGTPDMIGK FVDIKITDVYTNSLRGEVVRTEDEMGLRVVESAASVIARTRKEDDLGVGKYVVNL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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