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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP427875STCYeast1mgQuote
EP427875STCE. coli1mgQuote
BP427875STCBaculovirus200ugQuote
MP427875STCMammalian Cell200ugQuote

Protein Information

SpeciesSalinispora arenicola (strain CNS-205)
UniProt IDA8M7W6
Gene NamemiaB; Locus:Sare_1400
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-502
Expression Tag6xHis
Purity>90%
AA SequenceMVMTTAAASSPRTYQVRTYGCQMNVHDSERIAGLLEQAGYLRAGDADDVPDVVVFNTCAV RENADNRLYGNLGQLRPSKDRHPGMQIAVGGCLAQKDRSEIVRRAPWVDVVFGTHNIGSL PVLLERARHNAAAEVEILESLDVFPSTLPTRRESTYAGWVSISVGCNNTCTFCIVPALRG KERDRRPGDVLSEVRALVDEGVLEVTLLGQNVNSYGIEFGDRYAFGKLLRACGDIDGLER VRFTSPHPKDFTDDVIAAMAETPNVCHSLHMPLQSGSDDVLRAMRRSYRSERYLGIIEKV RAAMPDAAITTDIIVGFPGETEADFERTLDVVRSARFSSAFTFQYSKRPGTPAATMADQL PKPVVQERYERLVACVEEITWAENRRLVGETVEVLVAVGEGRKDERTGRLSGRARDGRLV HFDAGSLAGQIRPGDVVHTTVTYAAPHHLNADGEPLAHRRTPAGDAAEAGRRPRTAGVSL GLPTVGAPPSPVPPAASSACAC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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