Request QuoteCatalog Number: xP536284FFCSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP536284FFCYeast1mgQuote
EP536284FFCE. coli1mgQuote
BP536284FFCBaculovirus200ugQuote
MP536284FFCMammalian Cell200ugQuote

Protein Information

SpeciesFrancisella philomiragia subsp. philomiragia (strain ATCC 25017)
UniProt IDB0TXS4
Gene NamemiaB; Locus:Fphi_0023
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-442
Expression Tag6xHis
Purity>90%
AA SequenceMKEQKKVFIKTLGCQMNEYDSQKMHEVLDEHFHTVKTDDYKEADIILINTCSIREKAQEK VFHELGRWKNLKKKKEDLVIGVGGCVASQEGENIIKRAPFVDLVFGPQTIHRLPEMIKRK QSTQESQVDISFPEVEKFDFLPEPKAEGAKAYVSIMEGCDKYCSYCVVPYTRGPEVNRPF EDVLGECAALAEQGVKEITLLGQNVNHYLGPMENGETADLALLIHFIAEIDGIERIRFTT SHPVEFSQNLIDAYGSVPELANHLHLPVQHGSDRVLINMKRNHTILEFKQKIRKLRAIRP DITISSDFIVGFPGETEEDFQKLMDLVKDVNFDQSFSFIYSKRPGTPAADLPDDTPMEVK KDRLKRLQDLLNSNAQIISRQMVGTEQRILVEGTSKKDDNVLAGRTENNRIVNFIGDKSL IGQFAMVKITESLPNSLRGELI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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