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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP432762BOSYeast1mgQuote
EP432762BOSE. coli1mgQuote
BP432762BOSBaculovirus200ugQuote
MP432762BOSMammalian Cell200ugQuote

Protein Information

SpeciesBartonella tribocorum (strain CIP 105476 / IBS 506)
UniProt IDA9IMW7
Gene NamemiaB; Locus:BT_0250
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-458
Expression Tag6xHis
Purity>90%
AA SequenceMNKVNPKNTSPVAPKKVFIKTYGCQMNVYDSQRMTDSLSSQGYVTTQTPNDADLILVNTC HIREKAAEKLYSDLGRLRVMRQERTPDKPLTVGVTGCVAQAEGSEILRRAPIVDLVIGPQ MYHRLPELLEKAKQGKKIIETDYAVEDKFAHLPPHNKRAVRKRGVSAFLTVQEGCDKFCT FCVVPYTRGAEISRSVEQITEEARQLIEAGVKEITLLGQNVNGWHGQSADGKTWRLGDLL YHLAKLDGLKRLRYTTSHPRDMDDSLIAAHRDLDMLMPYLHLPVQSGSDRILKAMNRQHK SSYYLHLIEKIRAARPDIAFSGDFIVGFPGETDEDFEETIKLIQQVQYSSAYSFKYSPRP GTVGATMKNHVDESVKDARLQHLQVLLLEQQNTFLRSKIGQKTDVLIEKPGRHSGQMVGR SPWLLPVVVDTESSTGSVVEIHIKNASSNSFVGEMTNR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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