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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP434356HSKYeast1mgQuote
EP434356HSKE. coli1mgQuote
BP434356HSKBaculovirus200ugQuote
MP434356HSKMammalian Cell200ugQuote

Protein Information

SpeciesHerpetosiphon aurantiacus (strain ATCC 23779 / DSM 785)
UniProt IDA9AZY8
Gene NamemiaB; Locus:Haur_1047
Protein Name
Region Expressed1-438
Expression Tag6xHis
Purity>90%
AA SequenceMERNRYFVWTVGCQMNVSDSERLESALQGVGYTPAEQAEDADFIVLNSCSVRANAEEKII GKITDIQRIKRERPDTKIVLWGCMVGPNNQSIFKKKLPMVDHFVSPSAVDEVLALAPNPI YQLDEPALPVADWQVPPVNVHVPINYGCNMSCAYCVIPLRRGKERSRPMEEIAEEVRRIC ARGAKEITLLGQIVDSYGHDLPGRPDLADLLEYLHETPGLVRLRFLTSHPAFMSEKLLHT IARLPKVMPDINLPIQAGDDQLLKVMKRGYTVAKYTKLIERIREIIPNVSLSTDIIVGHP GETREMFERTLEMVENIRFDKVHIAAYSSRPGTKAADMELDPALAVEHGEKQYRRIALER LQERIATERNEECLGHEVEVLVEEFTKGKWRGRDRNNKLVFFEADGDWYGKVVNIHVTET RPWWLGGNLIGETLAIGA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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