Request QuoteCatalog Number: xP432560BPSSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP432560BPSYeast1mgQuote
EP432560BPSE. coli1mgQuote
BP432560BPSBaculovirus200ugQuote
MP432560BPSMammalian Cell200ugQuote

Protein Information

SpeciesBurkholderia multivorans (strain ATCC 17616 / 249)
UniProt IDA9AFF8
Gene NamemiaB; Locus:Bmul_0605, BMULJ_02656
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-457
Expression Tag6xHis
Purity>90%
AA SequenceMTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDSPEDADIILFNTCSVREKAQEKVF SDLGRVRELKEAKPDLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRT SGRAQVDITFPEIEKFDHLPPARVEGPTAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD VLTEVAGLADQGVREVTLLGQNVNAYRGALTAGSSEVADFATLIEYVADIPGIERIRYTT SHPKEFTQRLIDTYAKVPKLVNHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP DLSLSTDLIVGFPGETEEDFDKMMALVHDMRYDTSFSFIYSPRPGTPAANLHDDTPRDVK LKRLQLVQATIEENVARISQSMVGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLAA HPRLIGQMIDVKINHAYPHSLRGELVLVSDDASAATH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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