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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP532361LOQYeast1mgQuote
EP532361LOQE. coli1mgQuote
BP532361LOQBaculovirus200ugQuote
MP532361LOQMammalian Cell200ugQuote

Protein Information

SpeciesLeptospira biflexa serovar Patoc (strain Patoc 1 / Ames)
UniProt IDB0S9E2
Gene NamemiaB; Locus:LBF_1762
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-458
Expression Tag6xHis
Purity>90%
AA SequenceMNPTLTESQTLTPTIQLGKVYVETYGCQMNEYDSGIVKELFRKEHYETTNVVEESDIIFL NTCAVRENAHAKIYGRLQSLGYLKKKNPNLVIGVLGCMAQNLGEDLFHQELPLDLIVGPD NYRTLPELIQNIRNGEKDVQLTRLSRSETYDELEPKVVNGIQAFVTIMRGCNNFCTFCVV PYTRGRERSREPKSIIHEIKQLQEMGVKQVTLLGQNVNSYSYESYDFCALVESILKETTI ERVRFTSPHPKDFPDHLISLMAKEDRFSSQIHMPLQAGSSKVLRDMKRSYTKEEYLDLVK KIQSVIPDIGITSDIIVGFPGETEEEFLETLEVVKKVKFDMSYMFKYSEREGTIAKRKFI DDVPEEVKSRRLIELVELQTKISLEKNTSKIGKIFSILIENTSKKSKQELCGRSHCGRMV VFPIPEGMSQDLSDWIGKTVNVLIEQATSATLKGKLIV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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