Request QuoteCatalog Number: xP378746AUBSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP378746AUBYeast1mgQuote
EP378746AUBE. coli1mgQuote
BP378746AUBBaculovirus200ugQuote
MP378746AUBMammalian Cell200ugQuote

Protein Information

SpeciesAcidovorax citrulli (strain AAC00-1) (Acidovorax avenae subsp. citrulli)
UniProt IDA1TKI5
Gene NamemiaB; Locus:Aave_0875
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-449
Expression Tag6xHis
Purity>90%
AA SequenceMAKKVFVKTFGCQMNEYDSDKMVDVLNAAQGYEPTQDVEEADLILFNTCSVREKAQEKVF SDLGRIKHLKARGVKIGVGGCVASQEGDEIIKRAPYVDVVFGPQTLHRLPELLAQREALA RPQVDISFPEIEKFDHLPPARVEGASAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFDDVL VEVAGLADQGVKEVTLLGQNVNAYLGKMGGTAEVADFALLLEYVADITGIERIRFTTSHP NEFTQRLIDAYARIPKLVSHLHLPVQHGSDRILMAMKRGYTAMEYKSTIRKLRAIRPDLA MSSDFIVGFPGETEDDFGKMMKLIDDIHFDNSFSFIFSPRPGTPAASLHDDTPHEVKLRR LQELQAVINANIKSISESRVGTVQRILVEGASKRDGSELMGRTECNRVVNFAGHPRLVGQ MVDVTITEAKAYTLRGEVVTADHGALAAH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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