Request QuoteCatalog Number: xP391919MOKSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP391919MOKYeast1mgQuote
EP391919MOKE. coli1mgQuote
BP391919MOKBaculovirus200ugQuote
MP391919MOKMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK) )
UniProt IDA4TCM9
Gene NamemiaB; Locus:Mflv_3967
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-512
Expression Tag6xHis
Purity>90%
AA SequenceMLQQADGVSPDRSSCDTPAPRTFEVRTYGCQMNVHDSERLSGLLEQAGYQRAGAGVDADI VVFNTCAVRENADNKLYGNLSHLAPRKQADPQMQIAVGGCLAQKDRDAVLRKAPWVDVVF GTHNIGSLPTLLDRARHNRVAQVEIAESLREFPSALPASRESAYAAWVSISVGCNNTCTF CIVPSLRGKEVDRRPGDVLAEVQALVDQGVLEITLLGQNVNAYGVSFADPTEARDRGAFA KLLRACGRIDGLERVRFTSPHPAEFTDDVIEAMAQTSNVCPTLHMPLQSGSDRILRAMRR SYRADRYLGIIDRVRTAIPHAAITTDLIVGFPGETEEDFQATLDVVEAARFSSAFTFQYS KRPGTPAAELEGQIPKAVVSERYQRLIELQERISWEENRAQIGREVELLVATGEGRKDAA TARMSGRARDGRLVHFAPGALGADIRPGDIVVTTVTGAAPHHLIADAGPAEHRRTRAGDA HAEGRTPKTGVGLGMPGIGAPEPAPVTQGCAL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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