$90.00Catalog Number: PYT439-2ugSize: 2ug

M CSF Mouse

Macrophage-Colony Stimulating Factor Mouse Recombinant

Macrophage Colony Stimulating Factor Mouse Recombinant produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 156 amino acids and having a total molecular mass of 36.4 KD.
MCSF is purified by proprietary chromatographic techniques.

Accession

P07141

Amino acid sequence

MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP.

Biological Activity

The ED50,  calculated by the dose-dependant stimulation of the proliferation of murine M-NFS-60 indicator cells was found 0.91ng/ml, corresponding to a specific activity of 1MIU/mg.

Formulation

The protein (1mg/ml) was lyophilized with 5mM Tris, pH 8.0 + 100mM NaCl.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized M-CSF in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized M-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution MCSF should be stored at 4oC between 2-7 days and for future use below
-18oC.
Please prevent freeze-thaw cycles.

Synonyms

CSF-1, Lanimostim, MCSF, M-CSF.

Quantity

$90.00

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