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Recombinant 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (apgM1)

Recombinant 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (apgM1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP523001MSRYeast1mgQuote
EP523001MSRE. coli1mgQuote
BP523001MSRBaculovirus200ugQuote
MP523001MSRMammalian Cell200ugQuote

Protein Information

SpeciesMethanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
UniProt IDO27628
Gene NameapgM1; Locus:MTH_1591
Protein Name2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1
Region Expressed1-409
Expression Tag6xHis
Purity>90%
AA SequenceMKGVIMIIDGMADRPLEKLSGRTPLEAASTPNMDRIAESGINGIMDPIKPGVRAGSDTSH LSILGYDPYRVYTGRGPFEAAGVGLDVKPGDIAFRCNFATADEDMIITDRRAGRIRSGTS SIARTINSMTIEGFEDVEIIFRESTGHRAVLVLRGPGLGDKVSDADPKVEGKPPKKIKAL DGSPESRKTAEVLNRIVKESYEILRDHPVNTGRIERGEAPANIILPRGAGAVPHVEKFQE RYDLKAACIAETGLIKGIGHLTGMDVIDVEGATGGIDTDLESIKNAISSAIAADYDFLLI NIDGADEAGHDGDLEGKVRFIERVDSILGDLMGDDIYFILTADHSTPVSVMDHTGDPVPI AITGPEVRVDDVTSFSERAAAAGGLCRIRGSDVMDILLDLMNKKEKFGA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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