Request QuoteCatalog Number: xP489862SYSSize: 0.2-1mg

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Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB)

Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP489862SYSYeast1mgQuote
EP489862SYSE. coli1mgQuote
BP489862SYSBaculovirus200ugQuote
MP489862SYSMammalian Cell200ugQuote

Protein Information

SpeciesShewanella baltica (strain OS223)
UniProt IDB8EAX1
Gene NameglgB; Locus:Sbal223_3028
Protein Name1,4-alpha-glucan branching enzyme GlgB
Region Expressed1-743
Expression Tag6xHis
Purity>90%
AA SequenceMMTQANAYFYDGADVALLNGQYTDVFSLLGMHSANEGKALIVRCFLRNALSVDVISIKDG RKVASLDKVNEQGLFAGTLGRRVKPFLYLLRVEYPQCQLDIVDPYQFDSLLNSDDIYLFG EGSAERAYEFLGANWRQTQGVEGVHFCVWAPNAKRVSVVGDFNHWDDTRHVMRQHLANGL WELFLPNVVEGAHYKFDLVYQNGERHAKSDPMATQMECAPHNASIVPPKAHHSWNDTAWM SKRAATAWHKAPMSAYEVHLGSWRRKGEQGEQYLDYQDLIEQLIPYVKEQGFTHIELMPI SEFPFDGSWGYQPVGLYAPTHRFGDANGLKAFVDACHQAGIGIILDWVSAHFPKDPHGLV RFDGTCLYEHEDPRKGTHPDWDTLIYNYDRGEVRSFLLSNACYWLREFHFDGLRLDAVSS MLYLDYSREPGQWLPNTYGGRENLEAINFLQILNQRLYQAFPGICMIAEESTAFAGVTKP TDQQGLGFGFKWNMGWMNDSLSYLGRDPLYRQFHHHQLTFSLMYAYTEQFMLSVSHDEVV HGKGSLLHKIPGDDWQKFATLRAYYGFMWGHPGKKLLFMGCEFGQRNEWNHNQSLDWHLL AYEPHQGVQRWLKDLNHLYQAMPALSVQDYEGAGFSWLDCENSRDSIFTFVRYGLAGDAP LVFVINMTPQLHTGFRIGLPLAGDYREYLNSDSQIYGGSNQGNAGTVVAESLPWQGMAQS ALITVPPLGCLVIGPATGLAEAN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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