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Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2 (VIT_05s0020g04080)

Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2 (VIT_05s0020g04080) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP515273VFQYeast1mgQuote
EP515273VFQE. coli1mgQuote
BP515273VFQBaculovirus200ugQuote
MP515273VFQMammalian Cell200ugQuote

Protein Information

SpeciesVitis vinifera (Grape)
UniProt IDF6HDT7
Gene NameLocus:VIT_05s0020g04080
Protein Name1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 2
Region Expressed1-199
Expression Tag6xHis
Purity>90%
AA SequenceMGSFKDDREEVLQAWYMDDSDEDQRLPHHRDPKEFVSLDQLAKLGVLSWRLDADNYETDE ELKKIREARGYSYMDFCEVCPEKLPNYEEKIKNFFEEHLHTDEEIRYCVAGSGYFDVRDQ NDSWIRVWLKKGGMIVLPAGIYHRFTLDSNNYIKAMRLFVGDPVWTPFNRPHDNLPARQE YLEAFGQKEAANHVVDAAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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